Molecular Weight of Urokinase

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The inhibition of high and low molecular weight urokinase in plasma.

The rates of inhibition of high molecular weight (HMW) and low molecular weight (LMW) urokinase (UK) incubated in plasma or with purified antithrombin III (AT-III) were compared. Using a fibrinolytic assay system to determine residual biologic activity, polyacrylamide gel electrophoresis to demonstrate the formation of complexes, and selective immunoprecipitation techniques to identify the plas...

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Isolation from human plasma of a plasminogen activator identical to urinary high molecular weight urokinase.

Two different plasmatic plasminogen activators (PA) can be demonstrated after sodium dodecyl sulfate polyacrylamide gel electrophoresis of plasma freshly collected from resting volunteers, followed by transfer of the gels onto plasminogen-rich fibrin-agarose plates. These two PA are also present in plasmas deficient in coagulation Factor XI, Factor XII, prekallikrein, or high molecular weight-k...

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Immunochemical properties and molecular forms of urokinase.

1. By the double immunodiffusion technique, highly purified high molecular weight urokinase (HMW-UK; MW 53, 000, 124, 000 IU/mg protein) and low molecular weight urokinase (LMW-UK; MW 32, 000, 230, 000 IU/mg protein) revealed the same antigenicity, and their fibrinolytic activities were equally quenched with rabbit antisera against both enzymes. 2. Specific antisera against the functionally-act...

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ژورنال

عنوان ژورنال: Nature

سال: 1965

ISSN: 0028-0836,1476-4687

DOI: 10.1038/208894a0